Coinfection with Entamoeba polecki and Brachyspira hyodysenteriae in a pig with severe diarrhea

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Gastrointestinal infection with Brachyspira spp in a filly with chronic diarrhea: a case report

An eleven month old filly was referred to the Research and Teaching Hospital of the Faculty of Veterinary Medicine at the University of Tehran. The animal presented a three-week history of diarrhea and previous treatments had failed to effect a recovery. Based on the history, clinical findings and results of laboratory investigations, it was assumed that a gastrointestinal disorder was affectin...

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Development of a duplex PCR assay for detection of Brachyspira hyodysenteriae and Brachyspira pilosicoli in pig feces.

A duplex PCR (D-PCR) amplifying portions of the Brachyspira hyodysenteriae NADH oxidase gene and the B. pilosicoli 16S rRNA gene was developed and then tested on DNA extracted from 178 porcine fecal samples. The feces also underwent anaerobic culture and species-specific PCRs. Fecal extraction-D-PCR detected seven additional samples containing B. hyodysenteriae and five more containing B. pilos...

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Development of a serological ELISA using a recombinant protein to identify pig herds infected with Brachyspira hyodysenteriae.

Brachyspira hyodysenteriae is an anaerobic spirochaete that can induce swine dysentery (SD), a severe mucohaemorrhagic colitis in grower and fattener pigs. The aim of this study was to develop a serological ELISA for use as a screening method to detect evidence of herd infection. Bioinformatic analysis of the complete genome sequence of strain WA1 was used to identify genes predicted to encode ...

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Brachyspira hyodysenteriae: detection, identification and antibiotic susceptibility

101 samples of faeces, colonic mucosa and rectal swabs taken from 100 pigs (29 commercial herds) were cultivated on Trypticase Soy Agar with 5% of sheep blood, spectinomycin (200 mg/l), vancomycin (50 mg/l), rifampicin (12.5 mg/l) and colistin (12.5 mg/l). Plates were incubated in an anaerobic container at 37°C for 5–7 days. 25 samples (10 faeces, 15 scrapings of colonic mucosa) were examined b...

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ژورنال

عنوان ژورنال: Journal of Veterinary Diagnostic Investigation

سال: 2019

ISSN: 1040-6387,1943-4936

DOI: 10.1177/1040638719829800